HOPE fixation: a novel fixing method and paraffin-embedding technique for human soft tissues.
Author(s): Olert J, Wiedorn KH, Goldmann T, Kühl H, Mehraein Y, Scherthan H, Niketeghad F, Vollmer E, Müller AM, Müller-Navia J
Publication: Pathol Res Pract, 2001, Vol. 197, Page 823-6
PubMed ID: 11795830 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
The purpose of this study was to determine the effects of HOPE-fixation on the preservation of morphology, antigenicity, and nucleic acids in liver, appendix, kidney, spleen, and placental tissue.
Summary of Findings:
Fixation of liver tissue using the HOPE technique yielded sufficient preservation of morphological details and was comparable to formalin-fixation. However, the authors note that cutting HOPE-fixed, paraffin-embedded blocks requires a highly skilled individual and is more difficult than cutting FFPE blocks. IHC staining of HOPE-fixed appendix, detection of alkaline phosphatase activity in HOPE-fixed placental tissue, and FISH using HOPE-fixed kidney were each successful, but no direct comparison with FFPE tissue was shown. RNA that was visible on an agarose gel was successfully extracted from 3 5 uM sections of HOPE-fixed spleen and placental tissue that were up to 5 years old, but the same was not true of FFPE spleen and placental tissue. The authors report that PCR and RT-PCR were successful using DNA and RNA extracted from HOPE-fixed, paraffin-embedded tissue.
Biospecimens
Preservative Types
- Other Preservative
- Formalin
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Morphology H-and-E microscopy Morphology Macroscopic observation Protein Immunohistochemistry Glycoprotein Immunohistochemistry Protein Enzyme assay DNA FISH DNA PCR RNA RT-PCR RNA Electrophoresis Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation Formalin (buffered)
HOPE
Immunohistochemistry Specific Targeted peptide/protein E-selectin
VCAM
ICAM