DNA and RNA isolated from tissues processed by microwave-accelerated apparatus MFX-800-3 are suitable for subsequent PCR and Q-RT-PCR amplification.
Author(s): Bödör C, Schmidt O, Csernus B, Rajnai H, Szende B
Publication: Pathol Oncol Res, 2007, Vol. 13, Page 149-52
PubMed ID: 17607377 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of formalin fixation by immersion using the TCP 15 tissue processor versus by microwave in the MFX-800-3 on PCR and real-time PCR amplification.
Summary of Findings:
Amplification of beta-globin DNA was possible from all four specimens, but one gallbladder specimen showed less amplification when fixation was by immersion rather than microwave. No effect of fixation method was found on real-time PCR amplification of ABL mRNA.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
- Normal
- Other diagnoses
Platform:
Analyte Technology Platform RNA Real-time qRT-PCR DNA PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Method of fixative delivery Immersion
Microwaved
PCR Specific Targeted nucleic acid Beta-globin
Real-time qRT-PCR Specific Targeted nucleic acid ABL
Biospecimen Acquisition Biospecimen location Colon
Gallbladder
Liver
Preaquisition Diagnosis/ patient condition Chronic inflammation