Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Modified methods for preparation of cryostat sections of skeletal muscle.

Author(s): Wu JS, Hogan GR, Morris JD

Publication: Muscle Nerve, 1985, Vol. 8, Page 664-6

PubMed ID: 2414653 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if morphological quality and the efficacy of enzyme staining are affected by the temperature of isopentane used for snap-freezing muscle specimens. Muscle from 5 patients were snap frozen in isopentane that was pre-cooled using either dry ice (-75 degrees C), an ultra-low freezer (-100 degrees C) or liquid nitrogen (-160 degrees C). All tissue specimens were submersed in isopentane for 10 seconds, mounted with tragacanth gum, and sectioned with a cryostat before staining of sections with hematoxylin and eosin, modified trichrome, adenosine triphosphatase, NADH-tetrazolium reductase, succinic dehydrogenase, alkaline phosphatase, acid phosphatase, esterase, periodic acid-Schiff, crystal violet, cresyl violet, phosphorylase, or oil red.

   Summary of Findings:

   Muscle specimens frozen by immersion in isopentane pre-cooled with liquid nitrogen, dry ice, or in an ultra-low freezer yielded sections of comparable morphological quality and histochemical staining when assessed by light microscopy.

   Biospecimens

   • Tissue - Muscle (Skeletal)

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Morphology	H-and-E microscopy
   Protein	Enzyme assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Cooling or freezing method/ rate	Pre-cooled isopentane, multiple cooling methods examined

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