MicroRNA analysis as a potential diagnostic tool for papillary thyroid carcinoma.
Author(s): Chen YT, Kitabayashi N, Zhou XK, Fahey TJ 3rd, Scognamiglio T
Publication: Mod Pathol, 2008, Vol. 21, Page 1139-46
PubMed ID: 18587330 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to compare miRNA levels in FFPE surgical specimens with those in FNA which were snap-frozen in lysis buffer. RNA was extracted from tumor regions of 2-4 unstained sections using RecoverAll Total Nucleic Acid Isolation kit (Ambion). FNA were suspended in lysis buffer (Qiagen), snap frozen, and stored at -80 degrees C until RNA extraction using mirVana lysis buffer and phenol-chloroform extraction with columns.
Summary of Findings:
The RNA yields from snap-frozen FNA were lower than those from FFPE surgical specimens (0.41-12 ug versus 1.1-27 ug). The range of absorbance at 260/280 nm was larger for RNA from snap-frozen FNA than from FFPE surgical specimens (1.61-2.82 verses 1.90-2.12). Further, the CT values for amplification of U6 using RNA from snap-frozen FNA were higher than those obtained using RNA from FFPE surgical specimens. Patient diagnosis had a significant effect on the levels of miR-146b, -221, and -222.
Biospecimens
Preservative Types
- Formalin
- Frozen
Diagnoses:
- Normal
- Neoplastic - Benign
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform RNA Real-time qRT-PCR RNA Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Real-time qRT-PCR Specific Targeted nucleic acid miR-146a
miR-146b
miR-155
miR-187
miR221
miR-222
U6 RNA
Biospecimen Preservation Type of fixation/preservation Formalin (buffered)
Snap frozen
Preaquisition Diagnosis/ patient condition Normal thyroid
Hyperplastic nodules
Follicular adenoma
Classical papillary thyroid carcinoma
Follicular variant papillary thyroid carcinoma
Follicular carcinoma
Encapsulated follicular lesions
Biospecimen Acquisition Method of tissue acquisition Fine needle aspiration
Surgical resection