Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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MicroRNA analysis as a potential diagnostic tool for papillary thyroid carcinoma.

Author(s): Chen YT, Kitabayashi N, Zhou XK, Fahey TJ 3rd, Scognamiglio T

Publication: Mod Pathol, 2008, Vol. 21, Page 1139-46

PubMed ID: 18587330 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare miRNA levels in FFPE surgical specimens with those in FNA which were snap-frozen in lysis buffer. RNA was extracted from tumor regions of 2-4 unstained sections using RecoverAll Total Nucleic Acid Isolation kit (Ambion). FNA were suspended in lysis buffer (Qiagen), snap frozen, and stored at -80 degrees C until RNA extraction using mirVana lysis buffer and phenol-chloroform extraction with columns.

   Summary of Findings:

   The RNA yields from snap-frozen FNA were lower than those from FFPE surgical specimens (0.41-12 ug versus 1.1-27 ug). The range of absorbance at 260/280 nm was larger for RNA from snap-frozen FNA than from FFPE surgical specimens (1.61-2.82 verses 1.90-2.12). Further, the CT values for amplification of U6 using RNA from snap-frozen FNA were higher than those obtained using RNA from FFPE surgical specimens. Patient diagnosis had a significant effect on the levels of miR-146b, -221, and -222.

   Biospecimens

   • Tissue - Thyroid Gland

   Preservative Types

   • Formalin
   • Frozen

   Diagnoses:

   • Normal
   • Neoplastic - Benign
   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR
   RNA	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Real-time qRT-PCR Specific	Targeted nucleic acid	miR-146a miR-146b miR-155 miR-187 miR221 miR-222 U6 RNA
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) Snap frozen
   Preaquisition	Diagnosis/ patient condition	Normal thyroid Hyperplastic nodules Follicular adenoma Classical papillary thyroid carcinoma Follicular variant papillary thyroid carcinoma Follicular carcinoma Encapsulated follicular lesions
   Biospecimen Acquisition	Method of tissue acquisition	Fine needle aspiration Surgical resection

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