Comparison of p53 immunoreactivity in fresh-cut versus stored slides with and without microwave heating.

Author(s): Shin HJ, Kalapurakal SK, Lee JJ, Ro JY, Hong WK, Lee JS

Publication: Mod Pathol, 1997, Vol. 10, Page 224-30

PubMed ID: 9071730 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if p53 antigenicity is influenced by the duration of room temperature storage of paraffin blocks or slides with affixed formalin-fixed paraffin-embedded sections. The effectiveness of a microwave oven antigen retrieval technique was also investigated.

   Summary of Findings:

   p53 status (positive versus negative) was not influenced by the age of the paraffin block used for analysis, the duration of slide storage, or the application of microwave-mediated antigen retrieval. A small albeit significant reduction in the percentage of immuno-positive cells (<6% mean difference) was observed among stored slides in reference to freshly sectioned controls with and without antigen retrieval, when respective controls were compared. A significant reduction in staining intensity was also observed among stored slides compared to freshly sectioned controls, although intensity increased in both stored and fresh slides with antigen retrieval was employed. The time line associated with antigenicity loss was not explored.

   Biospecimens

   • Tissue - Head and Neck

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	4-25 y (block storage) Fresh (unspecified duration; slide storage) 6-48 months (slide storage)
   Analyte Extraction and Purification	Antigen retrieval	Microwave heating in 1% zinc sulfate Untreated

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