RNA quantitative analysis from fixed and paraffin-embedded tissues.

Author(s): Stanta G, Bonin S, Utrera R

Publication: Methods Mol Biol, 1998, Vol. 86, Page 113-9

PubMed ID: 9664461 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effect of FFPE specimens on the quantitative analysis of RNA.

   Summary of Findings:

   Although more cycles were needed to achieve amplification of RNA from FFPE specimens, there was a linear relationship between the log of amplified product and the amount of starting material, indicating no inhibition of PCR based on the extraction method used. Because the RNA was degraded, the linear relationship was generally present from 30-60 cycles, but must be determined for each amplicon independently.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Formalin
   • None (Fresh)

   Diagnoses:

   • Neoplastic - Other

   Platform:

   Analyte	Technology Platform
   RNA	Dot blot or slot blot
   RNA	RT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) None (fresh)
   Preaquisition	Diagnosis/ patient condition	Glioma

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