Population screening for glucose-6-phosphate dehydrogenase deficiencies in Isabel Province, Solomon Islands, using a modified enzyme assay on filter paper dried bloodspots.

Author(s): Kuwahata M, Wijesinghe R, Ho MF, Pelecanos A, Bobogare A, Landry L, Bugora H, Vallely A, McCarthy J

Publication: Malar J, 2010, Vol. 9, Page 223

PubMed ID: 20684792 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage of DBS, fresh blood assay type, and patient gender on measured levels of G6PD. To examine the effects of storage temperature and duration, commercial G6PD was added to blood at known levels before spotting. The spots were air-dried and stored in Zip-Lock bags with desiccant. To compare three G6PD assays, liquid EDTA blood was tested within 4 days of collection. For the gender comparison, finger-prick blood from 8541 people from the Solomon Islands was spotted on 3 MM Whatman paper and air-dried at ambient temperature. Once dried, blood spots were stored in Zip-Lock bags with desiccant at ambient temperatures.

   Summary of Findings:

   Regardless of storage temperature, after 1 day of storage of DBS, G6PD levels were comparable to initial levels, however, after 5 days of storage at room temperature or 37 degrees C or 10 days at 4 degrees C, G6PD levels had decreased by 5% of initial levels. The reagents used for assay could be stored for 2 weeks at ambient temperature without adverse effects on measurements. All three kits correctly diagnosed 14 individuals with G6PD levels in the normal range and 5 with deficiencies using liquid blood specimens. However, 1 specimen was determined to contain intermediate levels by the quantitative Trinity Biotech assay and by the WST8 assay, but was categorized as G6PD deficient by RDT assay. While moderate G6PD deficiency (<15% normal levels) was observed in 21.7% of males (843 of 3,880) and 19.1% of females (886 of 4,636), severe deficiency (<10% of normal levels) was more prevalent in males than females (10.9% versus 3.6%, p<0.0001).

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • Other Preservative
   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Enzyme assay
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Patient gender	Female Male
   Storage	Storage duration	1 day 5 days 10 days 14 days
   Storage	Storage temperature	4 degrees C Room temperature 37 degrees C
   Biospecimen Preservation	Type of fixation/preservation	Air-dried None (fresh)
   Clinical chemistry/auto analyzer Specific	Technology platform	Trinity Biotech assay WST8 assay RDT-Binax Now

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