A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples.
Author(s): Vincek V, Nassiri M, Nadji M, Morales AR
Publication: Lab Invest, 2003, Vol. 83, Page 1427
PubMed ID: 14563944 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to compare tissues preserved in universal molecular fixative (UMFIX) and then paraffin embedded with formalin fixed, paraffin-embedded (FFPE) and fresh frozen tissues. Adrenal, breast, colon, eye, esophagus, kidney, liver, lung, lymph node, skeletal muscle, pancreas, parathyroid, parotid, prostate, skin, small intestine, soft tissue, spleen, thyroid, tonsil, and uterus were investigated.
Summary of Findings:
The authors report that UMFIX adequately preserved histology (similar to FFPE tissues), RNA quality and quantity (similar to fresh-frozen tissues), and protein immunoreactivity (similar to FFPE tissues) when preserved immediately after excision; however RNA quality and quantity were adversely affected by delays in fixation with unpredictable RNA quality observed with a delay of 5 to 30 minutes, or failure to maintain an RNase-free environment. UMFIX-fixed paraffin embedded tissue stored at room temperature for 8 weeks yielded intact RNA.
Biospecimens
- Tissue - Adrenal Gland
- Tissue - Breast
- Tissue - Colorectal
- Tissue - Eye
- Tissue - Esophagus
- Tissue - Kidney
- Tissue - Liver
- Tissue - Lung
- Tissue - Lymph Node
- Tissue - Muscle (Skeletal)
- Tissue - Pancreas
- Tissue - Parathyroid Gland
- Tissue - Prostate
- Tissue - Skin
- Tissue - Small Bowel
- Tissue - Spleen
- Tissue - Thyroid Gland
- Tissue - Tonsil
- Tissue - Uterus
- Tissue - Salivary Gland
- Tissue - Other
Preservative Types
- Other Preservative
- Formalin
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform RNA Electrophoresis RNA Automated electrophoresis/Bioanalyzer Morphology H-and-E microscopy Protein Immunohistochemistry RNA Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation Universal molecular fixative (UMFIX)
Snap frozen
Formalin (buffered)
Storage Storage duration 1 day
4 weeks
8 weeks