Derivatized cellulose combined with MALDI-TOF MS: a new tool for serum protein profiling.
Author(s): Feuerstein I, Rainer M, Bernardo K, Stecher G, Huck CW, Kofler K, Pelzer A, Horninger W, Klocker H, Bartsch G, Bonn GK
Publication: J Proteome Res, 2005, Vol. 4, Page 2320-6
PubMed ID: 16335981 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of delayed processing, processing temperature and storage after processing on the proteome profiles of serum.
Conclusion of Paper
When serum was stored at room temperature for 4 h prior to protein extraction, all peaks over the 4000 m/z range were absent, but the spectra of serum did not change significantly during 3 weeks of storage of processed samples at -20 degrees C. The profiles obtained were similar when sample preparation was done at 4 or 21 degrees C, but profiles were different and there was more noise when preparation was performed at 30 degrees C. A very different profile was obtained from a single patient with cancer compared to a single normal control, but the authors report this to be unusual.
Studies
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Study Purpose
The purpose of this study was to determine the effects of delayed processing, processing temperature and storage of processed samples at -20 degrees C on the proteome profile of serum. Specimens were frozen at -80 degrees C and thawed up to three times including thawing for this study.
Summary of Findings:
When serum was stored at room temperature for 4 h prior to protein extraction, all peaks over the 4000 m/z range were absent, but the spectra of serum did not change significantly after 1 or 3 weeks of storage of processed samples at -20 degrees C compared to samples analyzed immediately after processing. The profiles obtained were similar when sample preparation was done at 4 or 21 degrees C, but profiles were different and there was more noise when preparation was performed at 30 degrees C. A very different profile was obtained from a single patient with cancer compared to a single normal control, but the authors report this to be unusual.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Neoplastic - Carcinoma
- Normal
Platform:
Analyte Technology Platform Protein MALDI-TOF MS Peptide MALDI-TOF MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 weeks
1 weeks
3 weeks
Storage Time at room temperature 0 h
4 h
Analyte Extraction and Purification Analyte purification 4 degrees C
21 degrees C
30 degrees C