Quantitative protein analysis form formalin-fixed tissues: implications for translational clinical research and nonscale molecular diagnosis

Author(s): Becker KF, Schott C, Hipp S, Metzger V, Porschewski P, Beck R, Nahrig J, Becker I, Hofler H

Publication: J Pathol, 2007, Vol. 211, Page 370

PubMed ID: 17133373 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to conduct a comparative analysis of tumor marker protein expression (E-cadherin, HER2, beta-actin, alpha-tubulin, GAPDH, snail, and a mutant E-cadherin with an exon 9 deletion) after protein extraction from FFPE and fresh frozen tissues, which included placenta, colon, gastric, breast, and pancreatic tissue.

   Summary of Findings:

   Quantities of full length immunoreactive proteins extracted from FFPE tissue were comparable to fresh frozen controls. While protein patterns from FFPE tissue were similar they were not identical to fresh frozen controls. All tumor markers examined (E-cadherin, HER2, beta-actin, alpha-tubulin, GAPDH, snail, and a mutant E-cadherin with an exon 9 deletion) were observed at comparable levels in FFPE and fresh frozen tissues as measured by Western blot analysis and reverse phase microarray.

   Biospecimens

   • Tissue - Breast
   • Tissue - Pancreas
   • Tissue - Stomach
   • Tissue - Placenta
   • Tissue - Colorectal

   Preservative Types

   • Formalin
   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma
   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Western blot
   Protein	1D/2D gels
   Protein	Reverse phase protein microarray

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) Snap frozen
   Western blot Specific	Targeted peptide/protein	E-cadherin HER-2 Beta-actin Alpha-tubulin GAPDH Snail

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