Evaluation and comparison of decalcification agents on the human teeth.

Author(s): Sanjai K, Kumarswamy J, Patil A, Papaiah L, Jayaram S, Krishnan L

Publication: J Oral Maxillofac Pathol, 2012, Vol. 16, Page 222-7

PubMed ID: 22923894 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of decalcifying agents on FFPE tooth specimen morphological parameters including ease of sectioning, hard-tissue staining, soft-tissue staining, soft-tissue attachment, soft-tissue shrinkage, and pulpal organization.

   Summary of Findings:

   Overall, for the decalcification of teeth, neutral EDTA yielded the best morphological results followed by 5% trichloroacetic acid. However, decalcification with EDTA required more time than decalcification with the other solutions. Overall, decalcification with Perenyi's fluid was inferior to the other methods and ranked the lowest for ease of sectioning, hard-tissue staining, soft-tissue attachment, soft-tissue cytoplasmic staining, and soft-tissue nuclear staining.

   Biospecimens

   • Tissue - Tooth

   Preservative Types

   • Formalin

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Morphology	H-and-E microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Analyte Extraction and Purification	Decalcification solution/ duration	5% nitric acid Perenyi's fluid Formalin-nitric acid solution EDTA 10% formic acid 5% trichloroacetic acid
   Biospecimen Acquisition	Biospecimen location	Incisor Canine Premolar Molar

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