Extensive postmortem stability of RNA from rat and human brain.

Author(s): Johnson SA, Morgan DG, Finch CE

Publication: J Neurosci Res, 1986, Vol. 16, Page 267

PubMed ID: 2427740 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to examine the stability of RNA in human brain cortex up to 36 hours postmortem.

   Summary of Findings:

   The authors report that although the total RNA isolated from postmortem brain appeared slightly degraded, the degree of degradation, as indicated by the size of beta-tubulin RNA, did not correlate with postmortem interval. They further report that in vitro translation products were of high-molecular-weight (> 90 kD) for all postmortem intervals although those from longer postmortem intervals exhibited somewhat reduced incorporation of radioactive sulfur, suggesting slight degradation of the corresponding mRNA.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Frozen
   • None (Fresh)

   Diagnoses:

   • Not specified
   • Autopsy

   Platform:

   Analyte	Technology Platform
   RNA	Northern blot
   Protein	1D/2D gels
   RNA	In vitro translation
   RNA	Electrophoresis

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Postmortem interval	7 h 15 h 24 h 36 h 0 h
   Biospecimen Preservation	Type of fixation/preservation	Snap frozen Fresh

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