Analysis and stability of retinol in plasma.
Author(s): Peng YM, Xu MJ, Alberts DS
Publication: J Natl Cancer Inst, 1987, Vol. 78, Page 95-9
PubMed ID: 3467133 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of anticoagulant, storage, and extraction method on retinol (ROH) levels in plasma and serum.
Conclusion of Paper
No difference between serum and herparin-plasma ROH levels were observed, but when EDTA was used as the anticoagulant a slight decrease in ROH recovery was observed. Storage of blood for up to 24 h, in the dark, on ice, prior to plasma separation, did not affect recovery of ROH. ROH in heparin-plasma was stable at -20 or -80 degrees C for at least 1 year. When ROH was extracted from spiked plasma, in the absence of perchloric acid, less than 50% of ROH was recovered; however, when perchloric acid was used, close to 100% recovery was attained. Once extracted from spiked plasma, ROH was stable for at least 4 h at room temperature, or 24 h at -20 degrees C, but decreased by 10% within the first hour of storage at 37 degrees C.
Studies
-
Study Purpose
The purpose of this study was to determine the effects of extraction method and storage of extracts on ROH recovery from spiked plasma specimens.
Summary of Findings:
When ROH was extracted from spiked plasma in the absence of perchloric acid, less than 50% of ROH was recovered; however, when perchloric acid was used for 30 seconds or 15 min, close to 100% recovery was attained. Once extracted from spiked plasma, ROH was stable for at least 4 h at room temperature but declined by 13% after 24 h. At -20 degrees C, extracted ROH was stable for at least 24 h. In contrast, 10% of ROH degraded within the first 1 h of storage at 37 degrees C.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Small molecule HPLC Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature Room temperature
-20 degrees C
37 degrees C
Storage Storage duration 0 h
1 h
2 h
4 h
24 h
Analyte Extraction and Purification Analyte isolation method Perchloric acid for 30 s
Perchloric acid for 15 min
No perchloric acid
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Study Purpose
The purpose of this study was to determine the effects of storage of blood or plasma and anticoagulant on endogenous ROH.
Summary of Findings:
No difference between serum and herparin-plasma ROH levels were observed, but when EDTA was used as the anticoagulant, a slight decrease in ROH was observed. The use of oxalate or citrate as anticoagulant also led to decreased ROH. Storage of blood for up to 24 h in the dark, on ice, prior to plasma separation, did not affect recovery of ROH. ROH in heparin-plasma was stable at -20 or -80 degrees C for at least 1 year.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Small molecule HPLC Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Anticoagulant Citrate
EDTA
Fluoride-oxalate
Lithium heparin
Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Whole blood
Storage Storage temperature -20 degrees C
-80 degrees C
On ice
Storage Storage conditions In the dark
Storage Storage duration 0 h
1 h
2 h
4 h
24 h
6 months
12 months
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated