IgH PCR of zinc formalin-fixed, paraffin-embedded non-lymphomatous gastric samples produces artifactual "clonal" bands not observed in paired tissues unexposed to zinc formalin
Author(s): Ahrens K, Braylan R, Almasri N, Foss R, Rimsza L
Publication: J Mol Diagn, 2002, Vol. 4, Page 159
PubMed ID: 12169677 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
To determine, retrospectively, if the nonreproducible bands indicative of ML but periodically observed in clinically diagnosed cases of HP gastritis are artifacts of zinc formalin fixation and paraffin embedding.
Summary of Findings:
IgH PCR analysis of gastric ML cases yielded monoclonal reproducible bands in 12 of the 16 cases examined. No false negatives were observed in zinc FFPE tissue samples. However, PCR analysis of HP gastritis resulted in distinct non reproducible bands (i.e. a single band of a fluctuating size) in zinc FFPE but not ethanol fixed samples.
Biospecimens
Preservative Types
- Other Preservative
- Ethanol
Diagnoses:
- Other diagnoses
Platform:
Analyte Technology Platform DNA PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation 50% ethanol
10% zinc formalin
-
Study Purpose
To determine if the nonreproducible bands observed after IgH PCR analysis in the FFPE HP gastritis cases are a result of nonspecific primer binding.
Summary of Findings:
Sequencing of the nonreproducible bands using primers spanning the IgH joining region confirmed they contained rearranged IgH sequence, indicating the amplicons are not due to nonspecific primer binding.
Biospecimens
Preservative Types
- Formalin
- Other Preservative
Diagnoses:
- Other diagnoses
Platform:
Analyte Technology Platform DNA DNA sequencing Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation Ethanol
Zinc formalin