Standardization of immunohistochemistry for formalin-fixed, paraffin-embedded tissue sections based on the antigen-retrieval technique: from experiments to hypothesis.

Author(s): Shi SR, Liu C, Taylor CR

Publication: J Histochem Cytochem, 2007, Vol. 55, Page 105

PubMed ID: 16982846 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if antigen retrieval (0.05% citraconic anhydride, pH 7.5, and microwave heating) of FFPE sections fixed for different durations (6 h- 30 d) yielded similar results for ER and cytokeratin (CK) antigens.

   Summary of Findings:

   All FFPE tissue subjected to antigen retrieval yielded appropriate positive immunostaining for ER and CK. The intensity of ER immunostaining was slightly reduced in specimens fixed for longer (30 d) compared to shorter durations. The authors conclude reported results support their hypothesis of antigen retrieval-mediated standardization of specimens fixed for different durations.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Time in fixative	6 h 12 h 24 h 3 d 7 d 14 d 30 d
   Immunohistochemistry Specific	Targeted peptide/protein	Estrogen receptor Cytokeratin

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