Proteome analysis of microdissected formalin-fixed and paraffin-embedded tissue specimens.

Author(s): Guo T, Wang W, Rudnick PA, Song T, Li J, Zhuang Z, Weil RJ, DeVoe DL, Lee CS, Balgley BM

Publication: J Histochem Cytochem, 2007, Vol. 55, Page 763-72

PubMed ID: 17409379 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare protein quality and profiles of FFPE and case-matched frozen glioblastoma specimens after manual dissection, detergent-based protein extraction, and heat-induced antigen retrieval.

   Summary of Findings:

   Snap-frozen and manually microdissected glioblastoma specimens yielded protein of superior quality compared to case-matched FFPE specimens. Heat-induced antigen retrieval combined with the highly sensitive separation strategy and mass spectrometry enabled the researchers to identify similar proteins in FFPE and fresh-frozen tissue. Of the 3902 proteins identified only 8.5% were unique to FFPE specimens, with an approximate 88% overlap among fixation methods.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Formalin
   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	LC-MS or LC-MS/MS
   Protein	1D/2D gels

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) Snap frozen

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