Routine acid decalcification of bone marrow samples can preserve DNA for FISH and CGH studies in metastatic prostate cancer.

Author(s): Brown RS, Edwards J, Bartlett JW, Jones C, Dogan A

Publication: J Histochem Cytochem, 2002, Vol. 50, Page 113-5

PubMed ID: 11748301 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of formic acid decalcification on DNA detection by FISH.

   Summary of Findings:

   Bone marrow specimens decalcified with 5% formic acid in water were suitable for FISH detection of the AR. When specimens were decalcified with 10% formic acid, no FISH signal was observed. Electrophoresis showed DNA fragments of up to 1500 bp were extractable from specimens decalcified with 5% formic acid indicating suitability for CGH.

   Biospecimens

   • Tissue - Bone Marrow

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   DNA	FISH
   DNA	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Analyte Extraction and Purification	Decalcification solution/ duration	5% formic acid for 12-18 h 10% formic acid for 7-10 days

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