Molecular detection of JC virus in embalmed, formalin-fixed, paraffin-embedded brain tissue.

Author(s): Amaker B H, Chandler Jr F W, Huey L O, Colwell R M

Publication: J Forensic Sci, 1997, Vol. 42, Page 1157

PubMed ID: 9397562 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to establish the feasibility of performing successful immunohistochemistry, in situ hybridization, and polymerase chain reaction in brain tissue that was embalmed approximately 24 h prior to autopsy, post-fixed in formalin, and paraffin-embedded.

   Summary of Findings:

   The authors report successfully detecting John Cunningham (JC) virus in embalmed, post-fixed tissue on each of the technological platforms tested. Sensitivity was comparable among in situ hybridization and immunohistochemistry platforms. They conclude that routine embalming should not dissuade researchers from considering use of the tissue.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Formalin
   • Other Preservative

   Diagnoses:

   • Autopsy
   • Leukoencephalopathy

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   DNA	Electrophoresis
   DNA	PCR
   RNA	In situ hybridization

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) Embalming

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