Tissues from routine pathology archives are suitable for microRNA analyses by quantitative PCR.

Author(s): Siebolts U, Varnholt H, Drebber U, Dienes HP, Wickenhauser C, Odenthal M

Publication: J Clin Pathol, 2009, Vol. 62, Page 84-8

PubMed ID: 18755714 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effect of method of preservation, tissue type and storage of FFPE blocks on expression of miR-16a in liver, breast, intestine, colon, lymph node, and bone marrow specimens.

   Summary of Findings:

   Expression of miR-16a was similar in unstored matched snap-frozen and FFPE colon and liver specimens. Absolute levels of miR-16 amplified from FFPE specimens varied slightly between tissue types (~5 fold variation) and between specimens from the same tissue type, which the authors note could be due to disease state. Generally, the highest levels of miR-16a were found in liver and intestine while the lowest were found in bone marrow, but even in bone marrow specimens, there was significant amplification of miR-16a. miR-16a levels in FFPE lymph node specimens that were stored for 17 or 27 years were approximately 40-50% of levels in recently obtained specimens (p<0.01). A non-significant reduction in miR-16a was found in blocks stored for 7 y compared to unstored blocks.

   Biospecimens

   • Tissue - Liver
   • Tissue - Colorectal
   • Tissue - Breast
   • Tissue - Bone Marrow
   • Tissue - Lymph Node
   • Tissue - Small Bowel

   Preservative Types

   • Formalin
   • Frozen

   Diagnoses:

   • Cirrhosis
   • Crohn's Disease
   • Hepatitis
   • Neoplastic - Carcinoma
   • Neoplastic - Benign
   • Ulcerative Colitis

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Snap frozen Formalin (buffered)
   Biospecimen Acquisition	Biospecimen location	Liver Colon Intestine Bone marrow Lymph node Breast
   Real-time qRT-PCR Specific	Targeted nucleic acid	miR-16a
   Storage	Storage duration	0 years 7 years 17 years 27 years

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