Glycolysis inhibitors negatively bias blood glucose measurements: potential impact on the reported prevalence of diabetes mellitus.
Author(s): Waring WS, Evans LE, Kirkpatrick CT
Publication: J Clin Pathol, 2007, Vol. 60, Page 820-3
PubMed ID: 17596547 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of NaF-KOx and storage on the measurement of glucose in blood specimens.
Summary of Findings:
Glucose concentrations were 4.7% lower when blood was collected in NaF-KOx tubes and stored 6.5-9.5 h rather than collected in serum gel tubes and separated within 15 min (p<0.0001). The difference in glucose concentrations between tubes was not affected by storage duration at room temperature (p=0.100). The authors believe the decrease in blood glucose concentrations found when blood was collected in NaF-KOx could alter the diabetes diagnosis rate.
Biospecimens
Preservative Types
- None (Fresh)
- Other Preservative
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 15 min
6.5-9.5 h
Biospecimen Acquisition Type of collection container/solution Serum gel tube
NaF-KOx tube
Biospecimen Preservation Type of fixation/preservation None (fresh)
Potassium oxalate
Sodium fluoride
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated