Immunohistochemical assays in prostatic biopsies processed in Bouin's fixative

Author(s): Ananthanarayanan V, Pins M R, Meyer R E, Gann P H

Publication: J Clin Pathol, 2005, Vol. 58, Page 322

PubMed ID: 15735170 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   Comparative analysis of immunohistochemical staining for several nuclear markers, (minichromosome maintenance protein 2 (MCM-2), p27, Ki-67), membrane markers (c-erb-B2), and CD-34 and alpha-methylacyl-CoA racemase (AMACR) in prostate biopsies fixed in either Bouins or formalin and paraffin embedded.

   Summary of Findings:

   While FFPE tissue displayed appropriate MCM-2 immunostaining, staining in BFPE tissue was negative and was unaltered by shortened fixation, lengthened primary antibody incubation, lengthened antigen retrieval, or methanol postfixation. P27 immunostaining was significantly reduced in BFPE compared to FFPE tissues, and included extensive nonspecific cytoplasmic staining that was minimal in FFPE samples. In BFPE samples, c-erb-B2 staining was strong and inappropriate in the majority of samples examined. However, immunostaining for AMACR, Ki-67, and CD-34 was comparable among BFPE and FFPE samples.

   Biospecimens

   • Tissue - Prostate

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Time in fixative	1 h 3 h 6 h 12 h
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) Bouins fixation

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2. Study Purpose

   Comparative analysis of Feulgen's stain for nuclear morphology in prostate biopsies fixed in either Bouins or formalin and paraffin embedded.

   Summary of Findings:

   Feulgen staining of nuclei was weak in BFPE compared to FFPE samples.

   Biospecimens

   • Tissue - Prostate

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Morphology	Feulgen's nuclear stain

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) Bouins fixative

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