Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Discordant quantitative detection of putative biomarkers in nodal micrometastases of colorectal cancer: biological and clinical implications.

Author(s): Kong SL, Salto-Tellez M, Leong AP, Chan YH, Koay ES

Publication: J Clin Pathol, 2005, Vol. 58, Page 839-44

PubMed ID: 16049286 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of preservation method and CRC stage on the expression of CEA, CK20, and GCC in lymph nodes.

   Summary of Findings:

   In Duke's B CRC specimens, discordant mRNA levels of CEA, CK20 and GCC were found in 33.3% (p=0.008), 33.3% (p=0.008) and 44.4% (p=0.001) of matched FFPE and frozen specimens. For Duke's C CRC specimens, CEA, CK20, and GCC levels were discordant in 7.7% (p=0.487), 23.1% (p=0.025) and 38.5% (p=0.000) of matched FFPE and frozen specimens. GCC was not amplified, and CEA and CK20 amplified at low levels in Duke's A CRC specimens. Only expression of CEA in FFPE and frozen specimens and GCC in frozen specimens correlated highly with Duke's classification (p=0.001, p=0.002, and p=0.012, respectively).

   Biospecimens

   • Tissue - Lymph Node

   Preservative Types

   • Formalin
   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) Snap frozen
   Preaquisition	Diagnosis/ patient condition	Duke's A colorectal cancer Duke's B colorectal cancer Duke's C colorectal cancer
   Real-time qRT-PCR Specific	Targeted nucleic acid	CEA CK20 GCC

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