Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

Search BRD Papers

An alternative protocol for DNA extraction from formalin fixed and paraffin wax embedded tissue.

Author(s): Coura R, Prolla J C, Meurer L, Ashton-Prolla P

Publication: J Clin Pathol, 2005, Vol. 58, Page 894

PubMed ID: 16049299 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   To evaluate the efficiency of PCR amplification using DNA extracted from formalin fixed paraffin embedded (FFPE) tissue sections after xylene/ethanol deparaffinization and overnight rehydration.

   Summary of Findings:

   PCR amplification of microsatellite markers (BAT-25, BAT-26, D2S123, D17S250) was successful in 100% of the paraffin embedded samples fixed in tamponated formalin. PCR amplification was unsuccessful, resulting in a smear, in all samples fixed in non tamponated formalin.

   Biospecimens

   • Tissue - Colorectal

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   DNA	PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Tamponated formalin Nontamponated formalin

   View more