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HIV-1 detection by nested PCR and viral culture in fresh or cryopreserved postmortem skin: potential implications for skin handling and allografting.

Author(s): Gala JL, Vandenbroucke AT, Vandercam B, Pirnay JP, Delferrière N, Burtonboy G

Publication: J Clin Pathol, 1997, Vol. 50, Page 481

PubMed ID: 9378813 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine if postmortem interval (PMI) or tissue type (skin, blood) impact HIV positivity assayed by viral culture and nested PCR for gag and pol sequences. Paired samples were used and the potential effects of cryopreservative method were also assessed.

Conclusion of Paper

The authors report that gag and pol sequences, or pol sequence, alone were detected by nested PCR in 100% of HIV-infected skin samples regardless of postmortem interval or whether the specimen had been cryopreserved. However, false negative results were obtained for 50% of HIV-infected blood specimens after a PMI of 2 or more days. The authors concluded that nested PCR detection of HIV is more reliable in postmortem skin specimens than in blood.

Studies

  1. Study Purpose

    The purpose of this study was to evaluate the ability to detect HIV-1 proviral DNA in paired skin specimens from HIV-infected cadavers at various times postmortem and in the presence or absence of cryopreservative.

    Summary of Findings:

    The authors report that gag and pol or pol alone genes were identified with nested PCR in all of the 73 skin specimens tested regardless of postmortem interval or cryopreservation.

    Biospecimens
    Preservative Types
    • Frozen
    • None (Fresh)
    Diagnoses:
    • Autopsy
    • AIDS/HIV-related
    Platform:
    AnalyteTechnology Platform
    DNA PCR
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Biospecimen Preservation Type of fixation/preservation No cryopreservation
    Cryopreserved in 5% dimethylsulphoxide
    Cryopreserved in 30% glycerol
    Preaquisition Postmortem interval 1 day
    2 days
    3 days
    4 days
    5 days
    View more
  2. Study Purpose

    The purpose of this study was to evaluate the ability to detect HIV-1 proviral DNA in paired blood specimens from HIV-infected cadavers at various times postmortem.

    Summary of Findings:

    Blood specimens collected from HIV-infected cadavers exhibited variable efficacy of PCR-based detection of gag and pol genes, with a efficacy of 75%, 50%, 20%, and 100% on postmortem day 1, 2, 3, 4, and 5, respectively. In those specimens which tested positive, PCR results were obtained for both gag and pol sequences from postmortem day 1 to 3 while specimens collected on or after day 4 only yielded positive results for the pol sequence. All blood samples tested positive for anti-HIV antibodies.

    Biospecimens
    Preservative Types
    • None (Fresh)
    Diagnoses:
    • Autopsy
    • AIDS/HIV-related
    Platform:
    AnalyteTechnology Platform
    Protein Western blot
    DNA PCR
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Preaquisition Postmortem interval 1 day
    2 days
    3 days
    4 days
    5 days
    View more

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