PCR detection of HIV proviral DNA (gag) in the brains of patients with AIDS: comparison between results using fresh frozen and paraffin wax embedded specimens.

Author(s): An SF, Ciardi A, Scaravilli F

Publication: J Clin Pathol, 1994, Vol. 47, Page 990-4

PubMed ID: 7829695 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare detection of HIV DNA and antigen in FFPE and frozen brain specimens with various fixation times and postmortem intervals.

   Summary of Findings:

   IHC and PCR detection of HIV were possible in specimens that had been exposed to formalin for up to 8 months and with PMI of up to 4 days. Nine of the 20 cases were positive by IHC. PCR of FFPE specimens resulted in 14 positives including all of the specimens that were positive by IHC and those with a positive neuropathological diagnosis. The use of frozen specimens for PCR identified all the cases found in FFPE specimens and one additional case in which neuropathological abnormalities were absent.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Formalin
   • Frozen

   Diagnoses:

   • AIDS/HIV-related
   • Autopsy
   • Cytomegalovirus
   • Neoplastic - Lymphoma
   • Leukoencephalopathy
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   DNA	PCR
   DNA	Southern blot
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Frozen Formalin (buffered)
   Biospecimen Preservation	Time in fixative	1 month 1.5 months 2 months 3 months 4 months 5 months 6 months 7 months 8 months
   Preaquisition	Postmortem interval	17 h 1 day 2 days 3 days 4 days

   View more