Use of capillary blood samples as a new approach for diagnosis of Dengue virus infection.

Author(s): Matheus S, Meynard JB, Lacoste V, Morvan J, Deparis X

Publication: J Clin Microbiol, 2007, Vol. 45, Page 887-90

PubMed ID: 17229857 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of using capillary blood specimens on filter paper instead of venous serum specimens for the detection of dengue virus by RT-PCR and ELISA. Capillary specimens were collected onto filter paper and held in a tube at room temperature until analysis. The authors do not describe storage of serum from venous specimens.

   Summary of Findings:

   85.4% of patients (111/130) had identical results (positive or negative) using capillary specimens on filter paper or venous serum specimens for the detection of dengue virus. Of the unmatched results, 14 were positive for dengue virus in venous serum but negative in capillary specimens, and 5 were positive in capillary specimens but negative in sera. The overall sensitivity and specificity of capillary blood filter paper analysis were 81.6% and 90.7%, respectively, with a positive predictive value of 92.5%. The sensitivity and specificity of the capillary collection method were higher during the acute phase of infection (days 0-4 after onset of fever) than during the convalescent phase (day 5 or more), however, RT-PCR of capillary specimens detected viral particles in 4/15 cases during the convalescent phase of infection that were negative by RT-PCR of venous serum. For analysis by RT-PCR only, the sensitivity and specificity of capillary specimen analysis compared to venous specimen analysis were 90.7% and 82.9%, respectively.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   RNA	RT-PCR
   Protein	Immunoassay
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	Dengue fever (acute phase) Dengue fever (convalescent phase) No dengue fever
   Biospecimen Aliquots and Components	Blood and blood products	Serum Whole blood
   RT-PCR Specific	Technology platform	Cell isolation and immunofluorescence assay ELISA
   Biospecimen Acquisition	Anatomical location of blood draw	Capillary Vein

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