Comparison of protein precipitation methods for sample preparation prior to proteomic analysis.

Author(s): Jiang L, He L, Fountoulakis M

Publication: J Chromatogr A, 2004, Vol. 1023, Page 317

PubMed ID: 14753699 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to evaluate the efficacy of plasma ultrafiltration and four different protein precipitation methods for two-dimensional (2-D) gel electrophoresis.

   Summary of Findings:

   Results of 2D analysis indicated no significant differences in the electrophoretic patterns of samples with reference to the method of protein purification compared to untreated controls. Although protein recovery was adequate for analysis, yields appeared to be greater in samples treated via ultrafiltration, or TC or acetone precipitation. The authors reported that fractionation with ammonium sulfate effectively removed albumin from the specimens, enabling increased signals from lower abundance components.

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	1D/2D gels

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Analyte Extraction and Purification	Analyte isolation method	Ammonium-sulfate fractionation No ammonium-sulfate fractionation
   Analyte Extraction and Purification	Analyte isolation method	Ammonium sulfate precipitation Ultrafiltration TCA precipitation Acetone precipitation Chloroform/methanol precipitation

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