Immunohistochemical detection of estrogen and progesterone receptor and HER2 expression in breast carcinomas: comparison of cell block and tissue block preparations.

Author(s): Williams SL, Birdsong GG, Cohen C, Siddiqui MT

Publication: Int J Clin Exp Pathol, 2009, Vol. 2, Page 476-80

PubMed ID: 19294006 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare ER, PR and HER-2 status of breast cancer specimens by IHC staining in FNA CB specimens fixed in ethanol with TB specimens fixed in formalin. Fixation of FNA CB specimens in ethanol was followed by formalin fixation.

   Summary of Findings:

   Positive IHC staining results for ER and PR were 90.4% and 93.9% concordant, respectively between CB and TB specimens. However, agreement between FNA CB and TB specimens was only obtained 73.3% of the time with respect to positive HER-2 status. When positive and negative results were evaluated together, HER-2 staining discrepancy occurred 44.1% of the time. 60% of discrepant cases showed false positive HER-2 staining in FNA CB specimens.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Ethanol
   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Ethanol Formalin (buffered)
   Biospecimen Acquisition	Method of tissue acquisition	Fine needle aspiration Surgical resection

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