Evaluation of platelet storage lesions in platelet concentrates stored for seven days.

Author(s): Singh H, Chaudhary R, Ray V

Publication: Indian J Med Res, 2003, Vol. 118, Page 243-6

PubMed ID: 14870797 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage and the addition of EDTA on platelet count and volume and metabolic parameters in platelet concentrates. Eighty pooled platelet concentrates were stored on a horizontal bed shaker at 20-24 degrees C and sampled aseptically.

   Summary of Findings:

   After storage of platelet concentrates for 7 days at 20-24 degrees C, there were insignificant declines in platelet counts and MPV. When platelet concentrates were incubated with EDTA for 1 h, there was a significant decline in platelet count and an increase in MPV. During storage of platelet concentrates, there were significant declines in pH and ATP after 5 and 7 days, respectively (p<0.05), and the PO2 increased significantly in specimens stored for 7 days (p<0.001). The authors also state that partial pressure carbon dioxide (pCO2) declined in specimens stored for 5 days or more.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Gas	Clinical chemistry/auto analyzer
   Small molecule	pH
   Cell count/volume	Hematology/ auto analyzer
   Small molecule	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	0 days 1 day 3 days 5 days 7 days
   Biospecimen Preservation	Fixative additive/buffer	EDTA None

   View more