Application of tissue microarray technology to the study of non-Hodgkin's and Hodgkin's lymphoma.

Author(s): Hedvat CV, Hegde A, Chaganti RS, Chen B, Qin J, Filippa DA, Nimer SD, Teruya-Feldstein J

Publication: Hum Pathol, 2002, Vol. 33, Page 968-74

PubMed ID: 12395368 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of IHC staining of TMA versus whole section slides to evaluate protein expression in lymphoma specimens. In addition, ISH and IHC methods were compared for the detection of EBV. 2-3 cores for each specimen were on the TMA. When discrepancies were noted between two cores on the same TMA, the authors report the higher value.

   Summary of Findings:

   Between 21 and 55 TMA and whole section slides were examined by IHC for each antibody. In almost all cases, CD30, cyclin-D1, CD5, CD3, and CD20 IHC staining results were the same on TMA and whole section slides. CD10 staining also showed 95% agreement between slide types. However, Bcl-2, CD23, and CD43 IHC staining results had lower concordance rates and agreement (K=0.66, 88% agreement; K=0.53, 86% agreement; and K=0.77, 88% agreement, respectively) between TMA and whole section slides. False negatives obtained by staining TMA sections accounted for 70% of the discrepant results. When the presence of EBV was detected by IHC staining for LMP1 and by ISH using probes for EBER-1 and EBER-2, similar results were obtained by ISH and IHC for 26 of 27 specimens. The discrepant case was EBV+ by IHC and EBV- by ISH.

   Biospecimens

   • Tissue - Lymph Node
   • Tissue - Spleen
   • Tissue - Tonsil
   • Tissue - Thymus Gland

   Preservative Types

   • Formalin
   • Other Preservative

   Diagnoses:

   • Normal
   • Neoplastic - Lymphoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   Protein	Tissue microarray
   RNA	In situ hybridization

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Biospecimen location	Lymph node Spleen Thymus Tonsil
   Immunohistochemistry Specific	Targeted peptide/protein	CD3 CD5 CD10 CD20 CD23 CD30 CD43 Bcl-2 Cyclin D1 LMP1
   In situ hybridization Specific	Targeted nucleic acid	EBER-1 EBER-2
   Biospecimen Aliquots and Components	Type of slide	Whole section Tissue microarray
   In situ hybridization Specific	Technology platform	Immunohistochemistry
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) B-3 fixative

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