Differential antigen preservation during tissue autolysis.

Author(s): Pelstring RJ, Allred DC, Esther RJ, Lampkin SR, Banks PM

Publication: Hum Pathol, 1991, Vol. 22, Page 237-41

PubMed ID: 1706304 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of holding tissue in a 37 degrees C normal saline water bath, prior to OCT or formalin-fixation, on the preservation of common cellular antigens in lymphoid tissue.

   Summary of Findings:

   Tissue necrosis progressed as the duration of incubation at 37 degrees C increased. Despite this, most antibodies showed positive staining after at least 12 hours of simulated warm ischemia time for both OCT and formalin-fixed tissue. The exception was anti-epithelial keratin-3 (AE-3), which did not stain formalin-fixed tissue sections at all. Staining was observed for CLA, UCHL-1, epithelial membrane antigen, and kappa and lambda light chain antibodies (cytoplasmic) in both formalin-fixed and OCT specimens after 72 hours of simulated warm ischemia time. L-26 and anti-epithelial keratin-1 (AE-1) staining were also seen in OCT specimens after 72 hours of simulated warm ischemia time. The antigens most affected by 37 degrees C incubation were Leu-2a and vimentin which were undetectable in OCT and formalin-fixed specimens, respectively, after 12 hours.

   Biospecimens

   • Tissue - Lymph Node

   Preservative Types

   • Formalin
   • OCT

   Diagnoses:

   • Other diagnoses
   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   Morphology	H-and-E microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) OCT
   Preaquisition	Diagnosis/ patient condition	Reactive lymphoid hyperplasia
   Immunohistochemistry Specific	Targeted peptide/protein	CLA UCHL-1 L-26 Kappa light chain Lambda light chain Anti-epithelial keratin-1 Anti-epithelial keratin-3 Epithelial membrane antigen Vimentin Leu-1 Leu-2a Leu-3a+b Leu-4 Leu-14
   Biospecimen Acquisition	Unspecified ischemia time	0 h 4 h 8 h 12 h 24 h 48 h 72 h

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