Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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DNA microextraction from dried blood spots on filter paper blotters: potential applications to newborn screening.

Author(s): McCabe ER, Huang SZ, Seltzer WK, Law ML

Publication: Hum Genet, 1987, Vol. 75, Page 213-6

PubMed ID: 3030923 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare DNA extracted from DBS and fresh blood and to determine the effects of DBS volume and storage on DNA yield and degradation. EDTA-blood was drawn from an unspecified number of individuals, and DNA was directly extracted, or blood was spotted onto Whatman paper and air-dried. Only a single specimen for each volume/storage duration is shown, but proof of hybridization is shown for specimens from 6-9 individuals.

   Summary of Findings:

   From a 50 µL DBS, 35 µg of DNA was obtained which was slightly less DNA than the amount obtained from 35 µL of fresh blood. DNA yield increased when the volume of the DBS was increased to 200 µL, but DNA yield decreased when the DBS was stored for 4.5 months instead of being analyzed fresh. Importantly, DNA from fresh and stored DBS was not degraded and was of sufficient quality for restriction digestion and Southern hybridization.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • Other Preservative
   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   DNA	Southern blot
   DNA	Electrophoresis

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	0 months 4.5 months
   Southern blot Specific	Targeted nucleic acid	Y chromosome Beta globin
   Biospecimen Aliquots and Components	Aliquot size/volume	35 µl 50 µl 100 µl 200 µl
   Biospecimen Preservation	Type of fixation/preservation	Air-dried None (fresh)

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