The influence of fixation on immunoperoxidase staining of plasmacells in paraffin sections of intestinal biopsy specimens.
Author(s): Bosman FT, Lindeman J, Kuiper G, van der Wal A, Kreunig J
Publication: Histochemistry, 1977, Vol. 53, Page 57-62
PubMed ID: 332671 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of fixative on IHC staining and morphological preservation of gastric and duodenal biopsy specimens. All specimens were fixed for 3 hours at room temperature and stored in 70% ethanol prior to analysis.
Summary of Findings:
All fixatives except sublimate-formaldehyde and 10% formalin-6% gluteraldehyde resulted in good morphological preservation. While sublimate-formaldehyde adequately preserved morphology, tissue fixed in 10% formalin-6% gluteraldehyde showed poor morphological preservation. The authors state that fixation for more than 20 h in sublimate-formaldehyde led to difficult sectioning. The best localization and intensity of IHC staining was observed after fixation with sublimate-formaldehyde, while the next best localization and intensity was observed in Bouin's-fixed specimens. No IHC staining was observed in tissues fixed in buffered methanol-formalin or 10% formalin-6% gluteraldehyde, and inconsistent staining was observed in 10% neutral buffered formalin-fixed specimens.
Biospecimens
Preservative Types
- Formalin
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein Immunohistochemistry Morphology Light microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation Formalin (buffered)
Bouin's fixative
Sublimate-formaldehyde
Formalin-gluteraldehyde
Zenker's fluid
Methanol-formalin
Periodate lysine paraformaldehyde
Immunohistochemistry Specific Targeted peptide/protein Immunoglobulin
Gastrin