Rapid microwave fixation--a comparative morphometric study.
Author(s): Kayser K, Stute H, Lübcke J, Wazinski U
Publication: Histochem J, 1988, Vol. 20, Page 347-52
PubMed ID: 3065304 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of microwave mediated fixation and type of fixative solution on the morphological preservation of lung and lymph node specimens.
Summary of Findings:
Morphology was best preserved when lung and lymph node tissue was fixed by microwave irradiation in either Tris buffer or low concentrations of formalin (0.5% and 1%). Conventional fixation for 24 h in room temperature neutral buffered formalin (7%) resulted in less clearly defined chromatin distribution and cell boundaries. Microwave irradiation in other solutions such as methanol, ethanol, or distilled water resulted in inadequate fixation, regardless of concentration. No statistically significant differences were observed in nuclear area or minimum nuclear diameter for conventional fixation or microwave fixation in Tris buffer or 1% buffered formalin.
Biospecimens
Preservative Types
- Formalin
- Other Preservative
- Ethanol
Diagnoses:
- Normal
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Morphology H-and-E microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Biospecimen location Lung
Lymph node
Biospecimen Preservation Type of fixation/preservation Acetone
Ethanol
Formalin (buffered)
Glutaraldehyde
Methanol
Biospecimen Preservation Fixative additive/buffer DMSO
Multiple concentrations evaluated
Sodium chloride
Tris buffer
Distilled water
Biospecimen Preservation Concentration of fixative 50% Methanol
80% Methanol
100% Methanol
0.5% Formalin
1% Formalin
7% Formalin
Biospecimen Preservation Method of fixative delivery Immersion
Microwaved
Biospecimen Preservation Time in fixative 2 x 3 min
24 h