Microwave applications in classical staining methods in formalin-fixed human brain tissue: a comparison between heating with microwave and conventional ovens.

Author(s): Feirabend HK, Ploeger S

Publication: Eur J Morphol, 1991, Vol. 29, Page 185-97

PubMed ID: 1726666 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to identify optimal lipid solvents for three neuroanatomical stains (Nissl, Kluver-Barrera, Haggqvist stains), and to assess potential differences associated with conventional versus microwave oven heating of formalin-fixed archival brain specimens.

   Summary of Findings:

   Optimal results were obtained with Kluver-Barrera and Haggqvist staining after lipid removal via a methanol/chloroform solution. The fat solvents investigated yielded similar results for Nissl staining. Heating sections via a conventional or microwave oven produced equivalent results for all stains assessed.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Formalin

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Morphology	Light microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Analyte Extraction and Purification	Cell/tissue permeabilization	No solvent Acetone Xylene 96% Ethanol Methanol Chloroform Methanol/chloroform (1:2)
   Analyte Extraction and Purification	Temperature of heat-induced retrieval	Microwave oven heating Conventional oven heating

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