Estimation of estrogen receptor content in fine-needle aspirates from breast cancer using the monoclonal antibody 1D5 and microwave oven processing: correlation with paraffin embedded and frozen sections determinations.

Author(s): Schmitt FC, Bento MJ, Amendoeira I

Publication: Diagn Cytopathol, 1995, Vol. 13, Page 347-51

PubMed ID: 8599924 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of antibody type and preservation method, and to compare surgically excised breast cancer specimens and FNA with respect to ER IHC staining results. The time period between fine needle aspiration and surgical excision ranged from 1-28 d. The ethanol-fixed FNA smears were processed in a microwave-oven.

   Summary of Findings:

   When a 5 class scoring system was used (-, +, ++, +++, ++++) more intense staining was generally observed using the 1D5 antibody for ER IHC staining of ethanol-fixed FNA and formalin-fixed surgical excision specimens than when the H222 antibody was used for ER IHC staining of snap-frozen sections. With ER staining in snap-frozen sections taken as the standard and a two class scoring system, immunostaining in FNA showed a sensitivity of 95.4% and a specificity of 100% when 31 specimens were examined, while ER staining of formalin-fixed surgical specimens showed 100% sensitivity and specificity. The one discrepant FNA case was non-interpretable due to high background staining but was positive in both the formalin-fixed and snap-frozen sections.

   Biospecimens

   • Tissue - Breast
   • Cell - Breast

   Preservative Types

   • Ethanol
   • Frozen
   • Other Preservative
   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   Morphology	Light microscopy
   Morphology	H-and-E microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Air-dried Ethanol Formalin (buffered) Snap frozen
   Immunohistochemistry Specific	Type of antibody	1D5 H222
   Biospecimen Acquisition	Method of tissue acquisition	Surgical resection Fine needle aspiration

   View more