NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Haemolysis affects insulin but not C-peptide immunoassay.

Author(s): O'Rahilly S, Burnett MA, Smith RF, Darley JH, Turner RC

Publication: Diabetologia, 1987, Vol. 30, Page 394-6

PubMed ID: 3315796 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of hemolysis and method of insulin separation on the measurement of insulin and c-peptide in plasma.

Conclusion of Paper

Measured insulin levels declined with increasing plasma hemoglobin levels, regardless of which assay was used to quantify the insulin. Generally, traumatized specimens with no visible hemolysis had insulin levels comparable to non-traumatized specimens, but 2 of the 19 specimens had reduced insulin levels. In contrast, plasma c-peptide levels were unaffected by hemolysis. Strong correlations were observed between insulin levels measured after different methods of insulin separation, but higher levels were measured after double-antibody rather than charcoal separation (15.1 versus 9.0 mU/L).

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of hemolysis and method of insulin separation on the measurement of insulin and c-peptide in plasma. Four lithium-heparin blood specimens were obtained from each of 19 fasting relatives of type 2 diabetes patients 2 h after the start of glucose infusion into the bloodstream. Specimens were hemolyzed by passage through a 23 gauge needle 0, 2, 3, or 4 times, after which plasma was obtained and stored at -20°C.

    Summary of Findings:

    Measured insulin levels declined with increasing plasma hemoglobin levels and hemolysis score, regardless of which assay was used to quantify the insulin. Generally, hemolyzed specimens with no visible hemolysis had insulin levels comparable to non-traumatized specimens, but 2 of the 19 specimens had reduced levels. In contrast, plasma c-peptide levels were unaffected by hemolysis. The insulin levels measured by the assays with different methods of insulin separation were strongly correlated (r2=0.76), but insulin was higher when separation was performed by the double-antibody rather than by the charcoal method (15.1 versus 9.0 mU/L).

    Biospecimens
    Preservative Types
    • Frozen
    Diagnoses:
    • Not specified
    Platform:
    AnalyteTechnology Platform
    Protein Radioimmunoassay
    Peptide Radioimmunoassay
    Protein Macroscopic observation
    Protein Immunoassay
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Analyte Extraction and Purification Analyte isolation method Charcoal separation
    Double-antibody separation
    Biospecimen Aliquots and Components Hemolysis Fine needle aspiration-induced
    Not induced

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