Influence of EDTA and heparin on lipopolysaccharide binding and cell activation, evaluated at single-cell level in whole blood.

Author(s): Brunialti MK, Kallás EG, Freudenberg M, Galanos C, Salomao R

Publication: Cytometry, 2002, Vol. 50, Page 14-8

PubMed ID: 11857593 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of anticoagulants on LPS binding and cell activation in blood specimens.

   Summary of Findings:

   In heparin-treated blood, membrane bound biotinyated LPS was detected after a 5 minute incubation and remained at high levels after a 360 min incubation. On the other hand, although membrane bound biotinylated LPS was detected at high levels after a 5 minute incubation in EDTA-treated blood, the percentage of biotinylated LPS bound cells decreased between 60 and 360 min of incubation time. The level of induction of TNF-alpha and CD69 by LPS was higher in heparin-treated blood than in EDTA-treated blood.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Flow cytometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	EDTA Heparin
   Analyte Extraction and Purification	Incubation duration/condition	5 min 60 min 120 min 180 min 240 min 360 min

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