Active glucagon-like peptide-1 (GLP-1): storage of human plasma and stability over time.

Author(s): Di Marino L, Griffo E, Maione S, Mirabella M

Publication: Clin Chim Acta, 2011, Vol. 412, Page 1693-4

PubMed ID: 21600891 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage of plasma at -70 degrees C and thaw temperature on GLP-1 activity. K3EDTA- and EDTA-plasma were thawed at 4 degrees C and room temperature, respectively, prior to assaying after specified storage durations.

   Summary of Findings:

   As long as K3EDTA-plasma was thawed at 4 degrees C, storage at -70 degrees C for up to 299 days had no significant effects on GLP-1 activity, regardless of whether aprotinin or the DPP-IV inhibitor, DPP4, was added. However, when EDTA-plasma was thawed at room temperature after frozen storage, significantly decreased GLP-1 activity was observed (up to -46% with aprotinin and -38% with DPP4). The authors state that the decreases were not strictly related to the duration of storage and do not note any significant differences between specimens with added aprotinin and those with DPP4.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Fixative additive/buffer	Aprotinin DPP4
   Storage	Storage duration	0 d 14 d 21 d 29 d 41 d 63 d 77 d 83 d 125 d 131 d 138 d 208 d 239 d 288 d 292 d 299 d
   Storage	Thaw temperature/condition	Room temperature 4 degrees C
   Biospecimen Acquisition	Anticoagulant	EDTA Potassium EDTA

   View more