Effect of long-term storage at -80 degrees C on the various lipid parameters in stored plasma samples.

Author(s): Devanapalli B, Bermingham MA, Mahajan D

Publication: Clin Chim Acta, 2002, Vol. 322, Page 179-81

PubMed ID: 12104099 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage at -80 degrees C on the lipid profile of EDTA-plasma from fasting volunteers. The same machines and operator were used for the two analysis performed 1 year apart.

   Summary of Findings:

   After storage for 1 year at -80 degrees C, TC declined by an average of 21% (p<0.0001), and Tg declined by an average of 42% (p<0.0001), with a larger decline in Tg found in specimens with initial concentrationsin fresh specimens of >2 mmol/L. In general, HDL-C was unaffected by storage at -80 degrees C, however, specimens with an initial HDL-C concentration of <1 mmol/L showed an increase with storage. When initial levels of Lp(a) were <300 mg/dL, there was a slight increase with storage at -80 degrees C (p<0.0001), and when initial levels were >300 mg/dL, there was a large decline in Lp(a) concentration with storage (>75%, p=0.0002).

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • Frozen
   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Lipid	Clinical chemistry/auto analyzer
   Lipoprotein	Clinical chemistry/auto analyzer
   Steroid	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Biomarker level	<2 mmol/L Tg >2 mmol/L Tg <55 mmol/L total cholesterol >55 mmol/L total cholesterol <1 mmol/L HDL-C >1 mmol/L HDL-C <300 mg/dL Lp(a) >300 mg/dL Lp(a)
   Biospecimen Preservation	Type of fixation/preservation	Frozen None (fresh)
   Storage	Storage duration	<2 h 1 year

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