Albumin determination in frozen urines--underestimated results.

Author(s): Erman A, Rabinov M, Rosenfeld J

Publication: Clin Chim Acta, 1988, Vol. 174, Page 255-61

PubMed ID: 3390955 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage temperature and duration on total protein and albumin levels and pH of urine specimens.

   Summary of Findings:

   Urinary albumin and protein levels were each significantly higher in specimens kept at 4 degrees C for 1 d compared to specimens kept at -20 degrees C for 1 d (each p<0.001), but albumin and protein levels were similar in urine stored for 7 d at -20 degrees C and 4 degrees C. Specimen pH was not significantly changed by storage of specimens at -20 degrees C for 7 d compared to fresh urine, but albumin levels were lower in the frozen specimens compared to corresponding fresh ones. pH and albumin levels in specimens stored for 7 d at 4 degrees C were comparable to levels in fresh urine. The authors determined, by radioimmunoassay, that freezing the urine specimens caused partial sedimentation of proteins including albumin, but this did not occur when urine was stored at 4 degrees C.

   Biospecimens

   • Bodily Fluid - Urine

   Preservative Types

   • Frozen
   • None (Fresh)
   • Other Preservative

   Diagnoses:

   • Not specified
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Protein	Spectrophotometry
   Protein	Radioimmunoassay
   Small molecule	pH

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Frozen None (fresh) Refrigeration
   Preaquisition	Diagnosis/ patient condition	Proteinuric
   Storage	Storage temperature	4 degrees C -20 degrees C
   Storage	Storage duration	0 d 1 d 7 d

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