Simultaneous measurement of 25 inflammatory markers and neurotrophins in neonatal dried blood spots by immunoassay with xMAP technology.

Author(s): Skogstrand K, Thorsen P, Nørgaard-Pedersen B, Schendel DE, Sørensen LC, Hougaard DM

Publication: Clin Chem, 2005, Vol. 51, Page 1854-66

PubMed ID: 16081507 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of protein extraction method and frozen storage on 25 inflammatory markers and neurotrophins in DBS from the Danish DBS specimen bank using multiplex-xMAP immunoassays.

   Summary of Findings:

   Extraction of protein for 60-90 min at 45 degrees C led to the highest signal, but the authors reported problems with bead agglutination that were not present when protein was extracted from specimens at 4 degrees C or room temperature. The concentrations of most analytes were unaffected by storage of DBS for 23 years at -20 degrees C, but concentrations of IL-1 beta, IL8, MMP-9, TREM-1, and NT-4 showed marked decreases when DBS were stored for 3 years or more, and SIL6ra, CRP, and BDNF showed marked decreases in DBS stored for 23 years.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	1 month 3 years 23 years
   Analyte Extraction and Purification	Analyte isolation method	30 min 60 min 90 min 120 min 4 degrees C Room temperature 45 degrees C
   Immunoassay Specific	Targeted peptide/protein	IL-1 beta IL8 MMP-9 TREM-1 NT-4 SIL6ra CRP BDNF

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