Preservation of urine for flow cytometric and visual microscopic testing.

Author(s): Kouri T, Vuotari L, Pohjavaara S, Laippala P

Publication: Clin Chem, 2002, Vol. 48, Page 900-5

PubMed ID: 12029006 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study conducted a comparative assessment of urine preservation methods for automated analysis and microscopic evaluation in a time course experiment.

   Summary of Findings:

   Bacterial counts were best preserved over the 3 days of storage by refrigeration at 4 degrees C, followed by the use of formaldehyde or BD C&S tubes containing borate-formate-sorbitol powder. Red blood cell preservation was slightly better in urine specimens collected in BD C&S tubes compared to other methods after 3 days. Preservation of white blood cells and urine casts were superior in urine specimens either collected in BD C&S tubes or stored at 4 degrees C.

   Biospecimens

   • Bodily Fluid - Urine

   Preservative Types

   • Other Preservative
   • None (Fresh)
   • Ethanol

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Flow cytometry
   Cell count/volume	Light microscopy
   Small molecule	Flow cytometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	Morning of arrival in lab Afternoon of arrival in lab 1 d after arrival in lab 3 d after arrival in lab
   Storage	Storage temperature	4 degrees C 20 degrees C
   Biospecimen Preservation	Type of fixation/preservation	Borate-formate-sorbitol Ethanol Formaldehyde None (fresh) Refrigeration

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