Effects of temperature on stability of blood homocysteine in collection tubes containing 3-deazaadenosine.
Author(s): Hill DM, Johnson LJ, Burns PJ, Neale AM, Harmening DM, Kenney AC
Publication: Clin Chem, 2002, Vol. 48, Page 2017-22
PubMed ID: 12406988 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
The purpose of this study was to determine the effects of stabilization and storage of EDTA-blood on Hcy concentrations in plasma from 59 individuals. At each time point, an aliquot of blood was removed and centrifuged, and the resulting plasma was stored at -80 degrees C until analysis.
Summary of Findings:
In 59 EDTA-blood specimens stored with 3DA, Hcy was stable for at least 72 h in the refrigerator and 6 h at room temperature, but Hcy increased within 3 h in EDTA-blood stored without 3DA at room temperature or in the refrigerator. Further, 17 specimens stored with 3DA in the refrigerator were assayed again after 1 week and showed only insignificant increases in Hcy concentration. The absolute change in Hcy concentration during storage of EDTA-blood without 3DA at room temperature was similar regardless of starting Hcy level resulting in larger percentage changes in specimens with lower initial Hcy concentrations.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
- Normal
Platform:
Analyte Technology Platform Small molecule HPLC Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature Refrigerated (2-8 degrees C)
Room temperature (20-25 degrees C)
Storage Storage duration 0 h
3 h
6 h
24 h
72 h
1 week
Preaquisition Biomarker level 0-5 umol/L Hcy
5-10 umol/L Hcy
10-15 umol/L Hcy
15-20 umol/L Hcy
20-25 umol/L Hcy
25-30 umol/L Hcy
Biospecimen Preservation Type of fixation/preservation None (fresh)
Refrigeration
Biospecimen Preservation Fixative additive/buffer 3-deazaadenosine
None
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated