Changes in oxygen measurements when whole blood is stored in iced plastic or glass syringes.

Author(s): Mahoney JJ, Harvey JA, Wong RJ, Van Kessel AL

Publication: Clin Chem, 1991, Vol. 37, Page 1244-8

PubMed ID: 1823532 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage of whole blood or plasma in glass or plastic syringes in an ice bath and storage of whole blood in room temperature plastic syringes on pO2 and pCO2. Plasma and whole blood specimens from 10 volunteers were tonometered with a mixture of oxygen, carbon dioxide and nitrogen prior to storage.

   Summary of Findings:

   There were no significant changes in pO2 when plasma or whole blood was stored in glass syringes in an ice bath for up to 60 minutes, regardless of initial oxygen concentration. The pO2 in whole blood stored in plastic syringes in an ice bath increased, but the magnitude of the increase was greatest when the initial pO2 was highest and the increase was insignificant in specimens that were tonometered to 60 mL/L. Plasma pO2 increased in all specimens stored for 30 min in plastic syringes in an ice bath, but the increase was largest when the initial pO2 was lowest. When whole blood was stored for 30 min in plastic syringes at room temperature, pO2 increased, but the increase was not significant in specimens tonometered to 140 mL/L. The authors report that there were no significant effects of storage of plasma or whole blood in plastic or glass syringes on the pCO2.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • Other Preservative
   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Gas	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	None (fresh) Refrigeration
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Whole blood
   Storage	Storage duration	0 min 30 min 60 min 90 min
   Storage	Type of storage container	Plastic syringe Glass syringe
   Biospecimen Aliquots and Components	Biospecimen components	140 mL/L oxygen 100 mL/L oxygen 60 mL/L oxygen
   Storage	Storage temperature	In an ice bath Room temperature

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