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Effects of storage time and temperature on measurement of small concentrations of albumin in urine.

Author(s): Osberg I, Chase HP, Garg SK, DeAndrea A, Harris S, Hamilton R, Marshall G

Publication: Clin Chem, 1990, Vol. 36, Page 1428-30

PubMed ID: 2387037 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of storage temperature and duration on total protein and albumin content and the pH of urine specimens from diabetic and healthy individuals.

Conclusion of Paper

For specimens with albumin excretion rates (AERs) equal to or greater than 30 ug/min, there were no significant differences in total protein or albumin content seen after storage at 4 or -20 degrees C, regardless of storage duration, compared with fresh specimens. For specimens with lower AERs, storage at 4 degrees C also did not produce any significant changes in total protein or albumin content, however storage at -20 degrees C for 2, 8, or 24 weeks significantly decreased AERs (p=0.01, 0.0001, and 0.0001, respectively) compared to fresh specimens. Likewise, storage at -20 degrees C for 2, 8, or 24 weeks significantly increased pH values, but these changes were clinically insignificant. No significant differences in AERs were observed between specimens from diabetics and those from nondiabetic controls or between overnight collection specimens and those collected after exercise.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of storage temperature and duration on total protein and albumin content and the pH of urine specimens from diabetic and healthy individuals. All specimens were mixed and centrifuged prior to analysis.

    Summary of Findings:

    For specimens with AERs equal to or greater than 30 ug/min, there were no significant differences in total protein or albumin content seen after storage at 4 or -20 degrees C, regardless of storage duration, compared with fresh specimens. For specimens with lower AERs, storage at 4 degrees C also did not produce any significant changes in total protein or albumin content, however storage at -20 degrees C for 2, 8, or 24 weeks significantly decreased AERs (p=0.01, 0.0001, and 0.0001, respectively) compared to fresh specimens. Likewise, storage at -20 degrees C for 2, 8, or 24 weeks significantly increased pH values, but these changes were clinically insignificant. No significant differences in AERs were observed between specimens from diabetics and those from nondiabetic controls or between overnight collection specimens and those collected after exercise.

    Biospecimens
    Preservative Types
    • None (Fresh)
    • Other Preservative
    • Frozen
    Diagnoses:
    • Diabetes Type 1
    • Normal
    Platform:
    AnalyteTechnology Platform
    Protein Radioimmunoassay
    Protein Colorimetric assay
    Small molecule pH
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Biospecimen Preservation Type of fixation/preservation Frozen
    None (fresh)
    Refrigeration
    Storage Storage temperature 4 degrees C
    -20 degrees C
    Storage Storage duration 0 d
    1 week
    2 weeks
    8 weeks
    24 weeks
    Biospecimen Acquisition Time of biospecimen collection After exercise
    Overnight collection
    Preaquisition Biomarker level <7.6 ug/min AER
    7.6-18.0 ug/min AER
    18.1-30.0 ug/min AER
    30.1-200 ug/min AER
    >201 ug/min AER
    View more

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