Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Influence of O.C.T. embedding compound on determinations of estrogen and progestin receptors in breast cancer.

Author(s): Pasic R, Djulbegovic B, Wittliff JL

Publication: Clin Chem, 1989, Vol. 35, Page 2317-9

PubMed ID: 2591050 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine whether OCT compound is capable of interfering with ER and PR binding analyses. Case-matched biopsies of breast tumor were snap-frozen on dry ice in the presence or absence of OCT. Specimens were stored at -86 degrees C for 1-2 d prior to analysis.

   Summary of Findings:

   Results of radioligand-binding assays for OCT-embedded and un-embedded case-matched controls were not significantly different for ER or PR, nor was either receptor status (threshold was set at 10 fmol/mg cytosol protein) altered by use of OCT for any of the specimens examined. Further, ER and PR binding capability and Kd values did not differ between 306 un-embedded biobanked specimens and 80 biopsies that were embedded in OCT, as determined by the ligand titration assay in both the presence and absence of sodium molybdate.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • OCT
   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Receptor binding

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Frozen OCT
   Receptor binding Specific	Reaction solution	Sodium molybdate

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