Stabilization of ascorbic acid in human plasma, and its liquid-chromatographic measurement.
Author(s): Margolis SA, Davis TP
Publication: Clin Chem, 1988, Vol. 34, Page 2217-23
PubMed ID: 3180412 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the stability of ascorbic acid (AA) in plasma supplemented with dithiothreitol (DTT).
Conclusion of Paper
AA was stable in DTT preserved AA spiked plasma stored frozen (1 specimen) or freeze dried (6 specimens), at -80 degrees C, for at least 57 weeks and remained within 1 standard deviation of original values for 80 weeks. In normal patient plasma specimens stored frozen at -80 degrees C, endogenous AA was stable for at least 52 days when DTT was added. In contrast, in a specimen without DTT, AA was not detectable after storage. Collection of the blood into tubes containing DTT did not impact AA detection when compared to levels in plasma. The authors report no deterioration of AA was found in DTT preserved AA spiked plasma for up to 18 h when stored at 21 degrees C and for several weeks when stored at 4 degrees C.
Studies
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Study Purpose
The purpose of this study was to determine the stability of endogenous and spiked AA in plasma supplemented with DTT during storage. The effect of adding DTT during blood collection was also examined.
Summary of Findings:
AA was stable in DTT preserved AA spiked plasma stored frozen (1 specimen) or freeze dried (6 specimens), at -80 degrees C, for at least 57 weeks and remained within 1 standard deviation of original values for 80 weeks. In normal patient plasma specimens stored frozen at -80 degrees C, endogenous AA was stable for at least 52 days when DTT was added. In contrast, in a specimen without DTT, AA was not detectable after storage. Collection of the blood into tubes containing DTT did not impact AA detection when compared to levels in plasma. The authors report no deterioration of AA was found in DTT preserved AA spiked plasma for up to 18 h when stored at 21 degrees C and for several weeks when stored at 4 degrees C.
Biospecimens
Preservative Types
- Frozen
- Other Preservative
Diagnoses:
- Not specified
- Normal
Platform:
Analyte Technology Platform Small molecule HPLC Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 h
18 h
24 h
2 weeks
4 weeks
6 weeks
12 weeks
27 weeks
57 weeks
66 weeks
80 weeks
23 days
32 days
51 days
52 days
Storage Storage temperature -80 degrees C
4 degrees C
21 degrees C
Biospecimen Preservation Type of fixation/preservation Lyophilized
Snap frozen
Dithiothreitol