Development of control material for hemoglobin analysis.

Author(s): Pornprasert S, Kongthai K, Waneesorn J, Jaiping K, Treesuwan K

Publication: Clin Chem Lab Med, 2012, Vol. 50, Page 351-356

PubMed ID: 22070221 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of lyophilization, duration of storage of lyophilized erythrocytes at -20 degrees C, and method of analysis on the levels of HbA, HbA2/E, and HbF in blood from healthy individuals and those with beta-thalassemia. EDTA-blood was collected and stored at 4 degrees C for up to 3 days prior to analysis by HPLC. Packed red blood cells were prepared at 4 degrees C, lyophilized, and stored at -20 degrees C. After storage, the cells were reconstituted with distilled water.

   Summary of Findings:

   Lyophilized hemoglobin stored at -20 degrees C for up to 3 months had similar chromatograms and only insignificant changes in the percentages of HbA, HbA2/E, and HbF from those observed in the refrigerated specimens prior to lyophilization, regardless of patient diagnosis. Strong correlations in HbA, HbA2/E, and HbF levels between the three technology platforms were observed (all three r>0.99).

   Biospecimens

   • Cell - Red Blood Cells

   Preservative Types

   • Other Preservative
   • Frozen

   Diagnoses:

   • Normal
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Protein	HPLC
   Protein	Low pressure liquid chromatography (LPLC)
   Protein	Capillary electrophoresis-MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	Beta thalessemia Normal
   Biospecimen Preservation	Type of fixation/preservation	Frozen Lyophilized Refrigeration
   Storage	Storage duration	1 day 15 days 30 days 3 months
   HPLC Specific	Technology platform	Capillary electrophoresis Low pressure liquid chromatography

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