Type B natriuretic peptide stability in frozen plasma.

Author(s): Daves M, Cemin R

Publication: Clin Chem Lab Med, 2007, Vol. 45, Page 1257-8

PubMed ID: 17848123 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to investigate the effects of freezing and storage for 30 d, freeze thaw cycling (0, 2, 5, or 10 cycles) and cardiovascular disease on the stability of BNP in plasma specimens without the addition of protease inhibitors.

   Summary of Findings:

   BNP in fresh specimens from 29 healthy individuals ranged from 18-133 ng/L with an average of 57 ng/L. After freezing the specimen and storing it for 30 days at -20 degrees C, no significant change in the mean BNP level for normal specimens was observed. In contrast, the measured BNP in 38 specimens from individuals with cardiovascular disease decreased from an average of 1183 ng/L in fresh specimens to 1108 ng/L (p<0.0001) in specimens frozen for 30 days. Freeze-thaw cycling had no significant effect on BNP levels in three normal specimens, but did result in a progressive decline in 11 specimens from individuals with cardiovascular disease (p=0.04). The authors conclude that at normal physiological levels, BNP is stable through freezing and storage of at least 30 days, and through at least 10 freeze thaw cycles, but at pathological levels, BNP levels decrease over time and with freezing.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen
   • None (Fresh)

   Diagnoses:

   • Cardiovascular Disease
   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Frozen None (fresh)
   Storage	Freeze/thaw cycling	0 cycles 2 cycles 5 cycles 10 cycles
   Preaquisition	Diagnosis/ patient condition	Normal Cardiovascular disease
   Storage	Storage duration	0 d 30 d

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